Composite

Part:BBa_K4007004:Design

Designed by: Jonatan Joel Aliaga Rodriguez   Group: iGEM21_Bolivia   (2021-10-06)


Ultrasensitive biosensing complex por arsenic (#1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
    Illegal BglII site found at 2442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3962
    Illegal AgeI site found at 4074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1652
    Illegal SapI.rc site found at 2285


Design Notes

The sequence for this part was artificially synthetized and was required to have as few repetitive sequences as possible. ArsR binding sites, RBS and terminators sequences were modified for meeting this requirement.

Source

Based on the constructs generated by Wang. et al [1] but with several modifications.

References

[1] Wan, X., Volpetti, F., Petrova, E., French, C., Maerkl, S. J., & Wang, B. (2019). Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals. Nature Chemical Biology, 15(5), 540-548. https://doi.org/10.1038/s41589-019-0244-3