Composite
Part:BBa_K4007004:Design
Designed by: Jonatan Joel Aliaga Rodriguez Group: iGEM21_Bolivia (2021-10-06)
Ultrasensitive biosensing complex por arsenic (#1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 198
Illegal BglII site found at 2442 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3962
Illegal AgeI site found at 4074 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1652
Illegal SapI.rc site found at 2285
Design Notes
The sequence for this part was artificially synthetized and was required to have as few repetitive sequences as possible. ArsR binding sites, RBS and terminators sequences were modified for meeting this requirement.
Source
Based on the constructs generated by Wang. et al [1] but with several modifications.
References
[1] Wan, X., Volpetti, F., Petrova, E., French, C., Maerkl, S. J., & Wang, B. (2019). Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals. Nature Chemical Biology, 15(5), 540-548. https://doi.org/10.1038/s41589-019-0244-3